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Superficial Bound of the Depth Limit of Two-Photon Imaging in Mouse Brain
Two-photon fluorescence microscopy has been used extensively to probe the structure and functions of cells in living biological tissue. Two-photon excitation generates fluorescence from the focal plane, but also from outside the focal plane, with out-of-focus fluorescence increasing as the focus is...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984806/ https://www.ncbi.nlm.nih.gov/pubmed/31907211 http://dx.doi.org/10.1523/ENEURO.0255-19.2019 |