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HAMA: a multiplexed LC-MS/MS assay for specificity profiling of adenylate-forming enzymes

Adenylation enzymes selecting substrates for ribosomal and nonribosomal protein and peptide biosynthesis have been popular targets of enzyme engineering. Previous standard assays for adenylation specificity have been cumbersome and failed to reflect the competition conditions inside a cell because t...

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Detalles Bibliográficos
Autores principales:	Stanišić, Aleksa, Hüsken, Annika, Kries, Hajo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Royal Society of Chemistry 2019
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988596/ https://www.ncbi.nlm.nih.gov/pubmed/32110329 http://dx.doi.org/10.1039/c9sc04222a

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