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Prompting Fab Yeast Surface Display Efficiency by ER Retention and Molecular Chaperon Co-expression
For antibody discovery and engineering, yeast surface display (YSD) of antigen-binding fragments (Fabs) and coupled fluorescence activated cell sorting (FACS) provide intact paratopic conformations and quantitative analysis at the monoclonal level, and thus holding great promises for numerous applic...
Autores principales: | Mei, Meng, Li, Junhong, Wang, Shengchen, Lee, Ki Baek, Iverson, Brent L., Zhang, Guimin, Ge, Xin, Yi, Li |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988814/ https://www.ncbi.nlm.nih.gov/pubmed/32039168 http://dx.doi.org/10.3389/fbioe.2019.00362 |
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