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Localization microscopy at doubled precision with patterned illumination
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field-of-view. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron size...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989044/ https://www.ncbi.nlm.nih.gov/pubmed/31819263 http://dx.doi.org/10.1038/s41592-019-0657-7 |