Localization microscopy at doubled precision with patterned illumination

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field-of-view. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron size...

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Detalles Bibliográficos
Autores principales: Cnossen, Jelmer, Hinsdale, Taylor, Thorsen, Rasmus Ø., Siemons, Marijn, Schueder, Florian, Jungmann, Ralf, Smith, Carlas S., Rieger, Bernd, Stallinga, Sjoerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989044/
https://www.ncbi.nlm.nih.gov/pubmed/31819263
http://dx.doi.org/10.1038/s41592-019-0657-7
Descripción
Sumario:MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field-of-view. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron sized field-of-view. We show a near twofold improvement in precision over standard localization with the same photon count on DNA-origami nano-structures and tubulin in cells, using DNA-PAINT and STORM imaging.

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