Localization microscopy at doubled precision with patterned illumination

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field-of-view. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron size...

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Autores principales: Cnossen, Jelmer, Hinsdale, Taylor, Thorsen, Rasmus Ø., Siemons, Marijn, Schueder, Florian, Jungmann, Ralf, Smith, Carlas S., Rieger, Bernd, Stallinga, Sjoerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989044/
https://www.ncbi.nlm.nih.gov/pubmed/31819263
http://dx.doi.org/10.1038/s41592-019-0657-7
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author Cnossen, Jelmer
Hinsdale, Taylor
Thorsen, Rasmus Ø.
Siemons, Marijn
Schueder, Florian
Jungmann, Ralf
Smith, Carlas S.
Rieger, Bernd
Stallinga, Sjoerd
author_facet Cnossen, Jelmer
Hinsdale, Taylor
Thorsen, Rasmus Ø.
Siemons, Marijn
Schueder, Florian
Jungmann, Ralf
Smith, Carlas S.
Rieger, Bernd
Stallinga, Sjoerd
author_sort Cnossen, Jelmer
collection PubMed
description MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field-of-view. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron sized field-of-view. We show a near twofold improvement in precision over standard localization with the same photon count on DNA-origami nano-structures and tubulin in cells, using DNA-PAINT and STORM imaging.
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spelling pubmed-69890442020-06-09 Localization microscopy at doubled precision with patterned illumination Cnossen, Jelmer Hinsdale, Taylor Thorsen, Rasmus Ø. Siemons, Marijn Schueder, Florian Jungmann, Ralf Smith, Carlas S. Rieger, Bernd Stallinga, Sjoerd Nat Methods Article MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field-of-view. Here, we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micron sized field-of-view. We show a near twofold improvement in precision over standard localization with the same photon count on DNA-origami nano-structures and tubulin in cells, using DNA-PAINT and STORM imaging. 2019-12-09 2020-01 /pmc/articles/PMC6989044/ /pubmed/31819263 http://dx.doi.org/10.1038/s41592-019-0657-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Cnossen, Jelmer
Hinsdale, Taylor
Thorsen, Rasmus Ø.
Siemons, Marijn
Schueder, Florian
Jungmann, Ralf
Smith, Carlas S.
Rieger, Bernd
Stallinga, Sjoerd
Localization microscopy at doubled precision with patterned illumination
title Localization microscopy at doubled precision with patterned illumination
title_full Localization microscopy at doubled precision with patterned illumination
title_fullStr Localization microscopy at doubled precision with patterned illumination
title_full_unstemmed Localization microscopy at doubled precision with patterned illumination
title_short Localization microscopy at doubled precision with patterned illumination
title_sort localization microscopy at doubled precision with patterned illumination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6989044/
https://www.ncbi.nlm.nih.gov/pubmed/31819263
http://dx.doi.org/10.1038/s41592-019-0657-7
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