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Improving the efficiency of gene insertion in a human artificial chromosome vector and its transfer in human-induced pluripotent stem cells

A human artificial chromosome (HAC) vector has potential to overcome the problems of stable gene expression associated with plasmid, transposon, and virus-based vectors, such as insertional mutagenesis, position effect, uncontrollable copy number, unstable gene expression, and DNA size limitation. T...

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Detalles Bibliográficos
Autores principales:	Hasegawa, Yoshinori, Ikeno, Masashi, Suzuki, Nobutaka, Nakayama, Manabu, Ohara, Osamu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Methods Manuscript
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994043/ https://www.ncbi.nlm.nih.gov/pubmed/32161806 http://dx.doi.org/10.1093/biomethods/bpy013

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