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A fast, sensitive and cost-effective method for nucleic acid detection using non-radioactive probes

Nucleic acid detection and quantification using a labeled DNA probe is a very common molecular biology procedure. Here, we describe a new method, based on commonly used laboratory solutions, for nucleic acid hybridization and detection with digoxigenin-labeled DNA probes. The protocol described is f...

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Detalles Bibliográficos
Autores principales: Viterbo, David, Marchal, Astrid, Mosbach, Valentine, Poggi, Lucie, Vaysse-Zinkhöfer, Wilhelm, Richard, Guy-Franck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994052/
https://www.ncbi.nlm.nih.gov/pubmed/32161800
http://dx.doi.org/10.1093/biomethods/bpy006