Covalent modification of primers improves PCR amplification specificity and yield

We report a method for covalent modification of primers that enhances the specificity of PCR and increases the yield of specific amplification products at the end of PCR. The introduction of thermally stable covalent modifications, such as alkyl groups to the exocyclic amines of deoxyadenosine or cy...

Descripción completa

Detalles Bibliográficos
Autores principales: Schoenbrunner, Nancy J, Gupta, Amar P, Young, Karen K Y, Will, Stephen G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Manuscript
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994073/
https://www.ncbi.nlm.nih.gov/pubmed/32161793
http://dx.doi.org/10.1093/biomethods/bpx011

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994073/
https://www.ncbi.nlm.nih.gov/pubmed/32161793
http://dx.doi.org/10.1093/biomethods/bpx011

Ejemplares similares