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A highly sensitive trap vector system for isolating reporter cells and identification of responsive genes

We devised a versatile vector system for efficient isolation of reporter cells responding to a certain condition of interest. This system combines nontoxic GAL4-UAS and piggyBac transposon systems, allowing application to mammalian cells and improved expression of a fluorescent reporter protein for...

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Detalles Bibliográficos
Autores principales: Ishikawa, Kosuke, Kobayashi, Yuta, Wakabayashi, Yutaro, Watanabe, Shinya, Semba, Kentaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994077/
https://www.ncbi.nlm.nih.gov/pubmed/32161797
http://dx.doi.org/10.1093/biomethods/bpy003