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A highly sensitive trap vector system for isolating reporter cells and identification of responsive genes

We devised a versatile vector system for efficient isolation of reporter cells responding to a certain condition of interest. This system combines nontoxic GAL4-UAS and piggyBac transposon systems, allowing application to mammalian cells and improved expression of a fluorescent reporter protein for...

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Detalles Bibliográficos
Autores principales:	Ishikawa, Kosuke, Kobayashi, Yuta, Wakabayashi, Yutaro, Watanabe, Shinya, Semba, Kentaro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Methods Manuscript
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994077/ https://www.ncbi.nlm.nih.gov/pubmed/32161797 http://dx.doi.org/10.1093/biomethods/bpy003

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