CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells

Discriminating between the mRNA and protein outputs of each of the alleles of an endogenous gene in intact cells, is a difficult task. To examine endogenous transcripts originating from a specific allele, we applied Central Dogma tagging (CD-tagging), which is based on a tag insertion into an endoge...

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Detalles Bibliográficos
Autores principales: Sheinberger, Jonathan, Hochberg, Hodaya, Lavi, Erez, Kanter, Itamar, Avivi, Shira, Reinitz, Gita, Schwed, Avital, Aizler, Yuval, Varon, Eli, Kinor, Noa, Shav-Tal, Yaron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Methods Manuscript
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994078/
https://www.ncbi.nlm.nih.gov/pubmed/32161787
http://dx.doi.org/10.1093/biomethods/bpx004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994078/
https://www.ncbi.nlm.nih.gov/pubmed/32161787
http://dx.doi.org/10.1093/biomethods/bpx004

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