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CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells
Discriminating between the mRNA and protein outputs of each of the alleles of an endogenous gene in intact cells, is a difficult task. To examine endogenous transcripts originating from a specific allele, we applied Central Dogma tagging (CD-tagging), which is based on a tag insertion into an endoge...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6994078/ https://www.ncbi.nlm.nih.gov/pubmed/32161787 http://dx.doi.org/10.1093/biomethods/bpx004 |