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A comparison of unamplified and massively multiplexed PCR amplification for murine antibody repertoire sequencing

Sequencing antibody repertoires has steadily become cheaper and easier. Sequencing methods usually rely on some form of amplification, often a massively multiplexed PCR prior to sequencing. To eliminate potential biases and create a data set that could be used for other studies, our laboratory compa...

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Detalles Bibliográficos
Autores principales: Rettig, Trisha A., Pecaut, Michael J., Chapes, Stephen K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6996338/
https://www.ncbi.nlm.nih.gov/pubmed/32123808
http://dx.doi.org/10.1096/fba.1017