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How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis

The breakthrough CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9-mediated genome-editing technology has led to great progress in monocot research; however, several factors need to be considered for the efficient implementation of this technology. To generate genome-edited crop...

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Detalles Bibliográficos
Autores principales: Yue, Jin-Jun, Hong, Chwan-Yang, Wei, Pengcheng, Tsai, Yu-Chang, Lin, Choun-Sea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997315/
https://www.ncbi.nlm.nih.gov/pubmed/32016561
http://dx.doi.org/10.1186/s12284-019-0354-2