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How to start your monocot CRISPR/Cas project: plasmid design, efficiency detection, and offspring analysis
The breakthrough CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9-mediated genome-editing technology has led to great progress in monocot research; however, several factors need to be considered for the efficient implementation of this technology. To generate genome-edited crop...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997315/ https://www.ncbi.nlm.nih.gov/pubmed/32016561 http://dx.doi.org/10.1186/s12284-019-0354-2 |