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Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing
BACKGROUND: Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. In order to make this system routine in plant biology, engineering efforts are needed for devel...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997322/ https://www.ncbi.nlm.nih.gov/pubmed/32016614 http://dx.doi.org/10.1186/s12284-020-0369-8 |