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CRISPR-PCDup: a novel approach for simultaneous segmental chromosomal duplication in Saccharomyces cerevisiae

In our previous study, a novel genome engineering technology, PCR-mediated chromosome duplication (PCDup), was developed in Saccharomyces cerevisiae that enabled the duplication of any desired chromosomal region, resulting in a segmental aneuploid. From one round of transformation, PCDup can duplica...

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Detalles Bibliográficos
Autores principales: Hassan, Naim, Sasano, Yu, Kimura, Shunta, Easmin, Farhana, Ekino, Keisuke, Taguchi, Hisataka, Harashima, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997328/
https://www.ncbi.nlm.nih.gov/pubmed/32016717
http://dx.doi.org/10.1186/s13568-020-0957-4