Significant improvement in catalytic activity and enantioselectivity of a Phaseolus vulgaris epoxide hydrolase, PvEH3, towards ortho-cresyl glycidyl ether based on the semi-rational design

The investigation of substrate spectrum towards five racemic (rac-) aryl glycidyl ethers (1a–5a) indicated that E. coli/pveh3, an E. coli BL21(DE3) transformant harboring a PvEH3-encoding gene pveh3, showed the highest EH activity and enantiomeric ratio (E) towards rac-3a. For efficiently catalyzing...

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Detalles Bibliográficos
Autores principales: Zhang, Chen, Liu, Youyi, Li, Chuang, Xu, Yaohui, Su, Yongjun, Li, Jinping, Zhao, Jun, Wu, Minchen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997370/
https://www.ncbi.nlm.nih.gov/pubmed/32015448
http://dx.doi.org/10.1038/s41598-020-58693-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6997370/
https://www.ncbi.nlm.nih.gov/pubmed/32015448
http://dx.doi.org/10.1038/s41598-020-58693-1

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