Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma

The current study aimed to explore the role of the circular RNA circ‐TCF4.85 and its downstream target microRNA‐486‐5p (miR‐486‐5p) in hepatocellular carcinoma (HCC) development. Circ‐TCF4.85 was detected to be highly expressed in HCC tissues. Next, we found that silencing of circ‐TCF4.85 repressed...

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Autores principales: Gao, Jun, Dai, Chao, Yu, Xin, Yin, Xiang‐Bao, Zhou, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6998385/
https://www.ncbi.nlm.nih.gov/pubmed/31758671
http://dx.doi.org/10.1002/1878-0261.12603
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author Gao, Jun
Dai, Chao
Yu, Xin
Yin, Xiang‐Bao
Zhou, Fan
author_facet Gao, Jun
Dai, Chao
Yu, Xin
Yin, Xiang‐Bao
Zhou, Fan
author_sort Gao, Jun
collection PubMed
description The current study aimed to explore the role of the circular RNA circ‐TCF4.85 and its downstream target microRNA‐486‐5p (miR‐486‐5p) in hepatocellular carcinoma (HCC) development. Circ‐TCF4.85 was detected to be highly expressed in HCC tissues. Next, we found that silencing of circ‐TCF4.85 repressed HCC cell proliferation, invasion, and migration, while enhancing apoptosis. In addition, biotin‐coupled probe pull‐down and miRNA capture assays, as well as fluorescence in situ hybridization, confirmed that circ‐TCF4.85 could bind to miR‐486‐5p. In rescue experiments, miR‐486‐5p had the potential to eliminate the tumor‐suppressive effects of circ‐TCF4.85 knockdown in HCC. Moreover, miR‐486‐5p was shown to target ABCF2 gene, which was positively regulated by circ‐TCF4.85. Finally, nude mice subcutaneously injected with si‐circ‐TCF4.85‐transfected HCC cells presented with inhibited xenograft tumor formation in vivo. Taken together, our results reveal that silencing of circ‐TCF4.85 suppresses HCC progression via miR‐486‐5p‐targeted inhibition of ABCF2.
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spelling pubmed-69983852020-02-05 Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma Gao, Jun Dai, Chao Yu, Xin Yin, Xiang‐Bao Zhou, Fan Mol Oncol Research Articles The current study aimed to explore the role of the circular RNA circ‐TCF4.85 and its downstream target microRNA‐486‐5p (miR‐486‐5p) in hepatocellular carcinoma (HCC) development. Circ‐TCF4.85 was detected to be highly expressed in HCC tissues. Next, we found that silencing of circ‐TCF4.85 repressed HCC cell proliferation, invasion, and migration, while enhancing apoptosis. In addition, biotin‐coupled probe pull‐down and miRNA capture assays, as well as fluorescence in situ hybridization, confirmed that circ‐TCF4.85 could bind to miR‐486‐5p. In rescue experiments, miR‐486‐5p had the potential to eliminate the tumor‐suppressive effects of circ‐TCF4.85 knockdown in HCC. Moreover, miR‐486‐5p was shown to target ABCF2 gene, which was positively regulated by circ‐TCF4.85. Finally, nude mice subcutaneously injected with si‐circ‐TCF4.85‐transfected HCC cells presented with inhibited xenograft tumor formation in vivo. Taken together, our results reveal that silencing of circ‐TCF4.85 suppresses HCC progression via miR‐486‐5p‐targeted inhibition of ABCF2. John Wiley and Sons Inc. 2020-01-10 2020-02 /pmc/articles/PMC6998385/ /pubmed/31758671 http://dx.doi.org/10.1002/1878-0261.12603 Text en © 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Gao, Jun
Dai, Chao
Yu, Xin
Yin, Xiang‐Bao
Zhou, Fan
Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma
title Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma
title_full Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma
title_fullStr Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma
title_full_unstemmed Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma
title_short Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma
title_sort circ‐tcf4.85 silencing inhibits cancer progression through microrna‐486‐5p‐targeted inhibition of abcf2 in hepatocellular carcinoma
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6998385/
https://www.ncbi.nlm.nih.gov/pubmed/31758671
http://dx.doi.org/10.1002/1878-0261.12603
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