Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma
The current study aimed to explore the role of the circular RNA circ‐TCF4.85 and its downstream target microRNA‐486‐5p (miR‐486‐5p) in hepatocellular carcinoma (HCC) development. Circ‐TCF4.85 was detected to be highly expressed in HCC tissues. Next, we found that silencing of circ‐TCF4.85 repressed...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6998385/ https://www.ncbi.nlm.nih.gov/pubmed/31758671 http://dx.doi.org/10.1002/1878-0261.12603 |
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author | Gao, Jun Dai, Chao Yu, Xin Yin, Xiang‐Bao Zhou, Fan |
author_facet | Gao, Jun Dai, Chao Yu, Xin Yin, Xiang‐Bao Zhou, Fan |
author_sort | Gao, Jun |
collection | PubMed |
description | The current study aimed to explore the role of the circular RNA circ‐TCF4.85 and its downstream target microRNA‐486‐5p (miR‐486‐5p) in hepatocellular carcinoma (HCC) development. Circ‐TCF4.85 was detected to be highly expressed in HCC tissues. Next, we found that silencing of circ‐TCF4.85 repressed HCC cell proliferation, invasion, and migration, while enhancing apoptosis. In addition, biotin‐coupled probe pull‐down and miRNA capture assays, as well as fluorescence in situ hybridization, confirmed that circ‐TCF4.85 could bind to miR‐486‐5p. In rescue experiments, miR‐486‐5p had the potential to eliminate the tumor‐suppressive effects of circ‐TCF4.85 knockdown in HCC. Moreover, miR‐486‐5p was shown to target ABCF2 gene, which was positively regulated by circ‐TCF4.85. Finally, nude mice subcutaneously injected with si‐circ‐TCF4.85‐transfected HCC cells presented with inhibited xenograft tumor formation in vivo. Taken together, our results reveal that silencing of circ‐TCF4.85 suppresses HCC progression via miR‐486‐5p‐targeted inhibition of ABCF2. |
format | Online Article Text |
id | pubmed-6998385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69983852020-02-05 Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma Gao, Jun Dai, Chao Yu, Xin Yin, Xiang‐Bao Zhou, Fan Mol Oncol Research Articles The current study aimed to explore the role of the circular RNA circ‐TCF4.85 and its downstream target microRNA‐486‐5p (miR‐486‐5p) in hepatocellular carcinoma (HCC) development. Circ‐TCF4.85 was detected to be highly expressed in HCC tissues. Next, we found that silencing of circ‐TCF4.85 repressed HCC cell proliferation, invasion, and migration, while enhancing apoptosis. In addition, biotin‐coupled probe pull‐down and miRNA capture assays, as well as fluorescence in situ hybridization, confirmed that circ‐TCF4.85 could bind to miR‐486‐5p. In rescue experiments, miR‐486‐5p had the potential to eliminate the tumor‐suppressive effects of circ‐TCF4.85 knockdown in HCC. Moreover, miR‐486‐5p was shown to target ABCF2 gene, which was positively regulated by circ‐TCF4.85. Finally, nude mice subcutaneously injected with si‐circ‐TCF4.85‐transfected HCC cells presented with inhibited xenograft tumor formation in vivo. Taken together, our results reveal that silencing of circ‐TCF4.85 suppresses HCC progression via miR‐486‐5p‐targeted inhibition of ABCF2. John Wiley and Sons Inc. 2020-01-10 2020-02 /pmc/articles/PMC6998385/ /pubmed/31758671 http://dx.doi.org/10.1002/1878-0261.12603 Text en © 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Gao, Jun Dai, Chao Yu, Xin Yin, Xiang‐Bao Zhou, Fan Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma |
title | Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma |
title_full | Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma |
title_fullStr | Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma |
title_full_unstemmed | Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma |
title_short | Circ‐TCF4.85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma |
title_sort | circ‐tcf4.85 silencing inhibits cancer progression through microrna‐486‐5p‐targeted inhibition of abcf2 in hepatocellular carcinoma |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6998385/ https://www.ncbi.nlm.nih.gov/pubmed/31758671 http://dx.doi.org/10.1002/1878-0261.12603 |
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