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Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching

Sulfobacillus and Leptospirillum occur frequently in leaching systems. Here we investigated the effects of cells of L. ferriphilum on biofilm formation and leaching performance by S. thermosulfidooxidans. The effects were caused by the presence of L. ferriphilum or an addition of pyrite leach liquor...

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Autores principales: Li, Qian, Zhu, Jianyu, Li, Shoupeng, Zhang, Ruiyong, Xiao, Tangfu, Sand, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000362/
https://www.ncbi.nlm.nih.gov/pubmed/32063894
http://dx.doi.org/10.3389/fmicb.2020.00044
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author Li, Qian
Zhu, Jianyu
Li, Shoupeng
Zhang, Ruiyong
Xiao, Tangfu
Sand, Wolfgang
author_facet Li, Qian
Zhu, Jianyu
Li, Shoupeng
Zhang, Ruiyong
Xiao, Tangfu
Sand, Wolfgang
author_sort Li, Qian
collection PubMed
description Sulfobacillus and Leptospirillum occur frequently in leaching systems. Here we investigated the effects of cells of L. ferriphilum on biofilm formation and leaching performance by S. thermosulfidooxidans. The effects were caused by the presence of L. ferriphilum or an addition of pyrite leach liquor from L. ferriphilum. Data show that the number of attached S. thermosulfidooxidans on pyrite increases, if the pyrite had been pre-colonized by living biofilms of L. ferriphilum, while it decreases if the pre-colonized biofilms had been inactivated. Coaggregation between S. thermosulfidooxidans and L. ferriphilum occurs during the dual-species biofilm formation, but different effects on bioleaching were noted, if the preculture of L. ferriphilum had been different. If L. ferriphilum had been pre-colonized on a pyrite, significantly negative effect was shown. However, if the two species were simultaneously inoculated into a sterile leaching system, the bioleaching efficiency was better than that of a pure culture of S. thermosulfidooxidans. The effect might be related to a metabolic preference of S. thermosulfidooxidans. If S. thermosulfidooxidans performed leaching in a filtered pyrite leachate from L. ferriphilum, the cells preferred to oxidize RISCs instead of ferrous ion and the number of attached cells decreased compared with the control. This study gives an indication that in a short-term multi-species leaching system the role of S. thermosufidooxidans may be related to the time of its introduction.
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spelling pubmed-70003622020-02-14 Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching Li, Qian Zhu, Jianyu Li, Shoupeng Zhang, Ruiyong Xiao, Tangfu Sand, Wolfgang Front Microbiol Microbiology Sulfobacillus and Leptospirillum occur frequently in leaching systems. Here we investigated the effects of cells of L. ferriphilum on biofilm formation and leaching performance by S. thermosulfidooxidans. The effects were caused by the presence of L. ferriphilum or an addition of pyrite leach liquor from L. ferriphilum. Data show that the number of attached S. thermosulfidooxidans on pyrite increases, if the pyrite had been pre-colonized by living biofilms of L. ferriphilum, while it decreases if the pre-colonized biofilms had been inactivated. Coaggregation between S. thermosulfidooxidans and L. ferriphilum occurs during the dual-species biofilm formation, but different effects on bioleaching were noted, if the preculture of L. ferriphilum had been different. If L. ferriphilum had been pre-colonized on a pyrite, significantly negative effect was shown. However, if the two species were simultaneously inoculated into a sterile leaching system, the bioleaching efficiency was better than that of a pure culture of S. thermosulfidooxidans. The effect might be related to a metabolic preference of S. thermosulfidooxidans. If S. thermosulfidooxidans performed leaching in a filtered pyrite leachate from L. ferriphilum, the cells preferred to oxidize RISCs instead of ferrous ion and the number of attached cells decreased compared with the control. This study gives an indication that in a short-term multi-species leaching system the role of S. thermosufidooxidans may be related to the time of its introduction. Frontiers Media S.A. 2020-01-29 /pmc/articles/PMC7000362/ /pubmed/32063894 http://dx.doi.org/10.3389/fmicb.2020.00044 Text en Copyright © 2020 Li, Zhu, Li, Zhang, Xiao and Sand. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Qian
Zhu, Jianyu
Li, Shoupeng
Zhang, Ruiyong
Xiao, Tangfu
Sand, Wolfgang
Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching
title Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching
title_full Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching
title_fullStr Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching
title_full_unstemmed Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching
title_short Interactions Between Cells of Sulfobacillus thermosulfidooxidans and Leptospirillum ferriphilum During Pyrite Bioleaching
title_sort interactions between cells of sulfobacillus thermosulfidooxidans and leptospirillum ferriphilum during pyrite bioleaching
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000362/
https://www.ncbi.nlm.nih.gov/pubmed/32063894
http://dx.doi.org/10.3389/fmicb.2020.00044
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