Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings
BACKGROUND: Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings remains poorly understood. To study the genetic regulatory network for miRNA-mRNA and the signaling pathways involve...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001231/ https://www.ncbi.nlm.nih.gov/pubmed/32019511 http://dx.doi.org/10.1186/s12864-020-6539-7 |
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author | Wu, Fengyao Lu, Fengying Fan, Xin Chao, Jin Liu, Chuanmin Pan, Qunxing Sun, Huawei Zhang, Xiaofei |
author_facet | Wu, Fengyao Lu, Fengying Fan, Xin Chao, Jin Liu, Chuanmin Pan, Qunxing Sun, Huawei Zhang, Xiaofei |
author_sort | Wu, Fengyao |
collection | PubMed |
description | BACKGROUND: Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings remains poorly understood. To study the genetic regulatory network for miRNA-mRNA and the signaling pathways involved in DHAV-3 infection in ducklings, we conducted global miRNA and mRNA expression profiling of duckling liver tissues infected with lethal DHAV-3 by high-throughput sequencing. RESULTS: We found 156 differentially expressed miRNAs (DEMs) and 7717 differentially expressed genes (DEGs) in livers of mock-infected and DHAV-3-infected duckling. A total of 19,606 miRNA-mRNA pairs with negatively correlated expression patterns were identified in miRNA-mRNA networks constructed on the basis of these DEMs and DEGs. Moreover, immune-related pathways, including the cytokine-cytokine receptor interaction, apoptosis, Toll-like receptor, Jak-STAT, and RIG-I-like receptor signaling pathway, were significantly enriched through analyzing functions of mRNAs in the network in response to DHAV-3 infection. Furthermore, apl-miR-32-5p, apl-miR-125-5p, apl-miR-128-3p, apl-miR-460-5p, and novel-m0012-3p were identified as potential regulators in the immune-related signaling pathways during DHAV-3 infection. And some host miRNAs were predicted to target the DHAV-3 genome. CONCLUSIONS: This is the first integrated analysis of miRNA and mRNA in DHAV-3-infected ducklings. The results indicated the important roles of miRNAs in regulating immune response genes and revealed the immune related miRNA-mRNA regulation network in the DHAV-3-infected duckling liver. These findings increase our knowledge of the roles of miRNAs and their target genes in DHAV-3 replication and pathogenesis. They also aid in the understanding of host-virus interactions. |
format | Online Article Text |
id | pubmed-7001231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-70012312020-02-10 Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings Wu, Fengyao Lu, Fengying Fan, Xin Chao, Jin Liu, Chuanmin Pan, Qunxing Sun, Huawei Zhang, Xiaofei BMC Genomics Research Article BACKGROUND: Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings remains poorly understood. To study the genetic regulatory network for miRNA-mRNA and the signaling pathways involved in DHAV-3 infection in ducklings, we conducted global miRNA and mRNA expression profiling of duckling liver tissues infected with lethal DHAV-3 by high-throughput sequencing. RESULTS: We found 156 differentially expressed miRNAs (DEMs) and 7717 differentially expressed genes (DEGs) in livers of mock-infected and DHAV-3-infected duckling. A total of 19,606 miRNA-mRNA pairs with negatively correlated expression patterns were identified in miRNA-mRNA networks constructed on the basis of these DEMs and DEGs. Moreover, immune-related pathways, including the cytokine-cytokine receptor interaction, apoptosis, Toll-like receptor, Jak-STAT, and RIG-I-like receptor signaling pathway, were significantly enriched through analyzing functions of mRNAs in the network in response to DHAV-3 infection. Furthermore, apl-miR-32-5p, apl-miR-125-5p, apl-miR-128-3p, apl-miR-460-5p, and novel-m0012-3p were identified as potential regulators in the immune-related signaling pathways during DHAV-3 infection. And some host miRNAs were predicted to target the DHAV-3 genome. CONCLUSIONS: This is the first integrated analysis of miRNA and mRNA in DHAV-3-infected ducklings. The results indicated the important roles of miRNAs in regulating immune response genes and revealed the immune related miRNA-mRNA regulation network in the DHAV-3-infected duckling liver. These findings increase our knowledge of the roles of miRNAs and their target genes in DHAV-3 replication and pathogenesis. They also aid in the understanding of host-virus interactions. BioMed Central 2020-02-04 /pmc/articles/PMC7001231/ /pubmed/32019511 http://dx.doi.org/10.1186/s12864-020-6539-7 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wu, Fengyao Lu, Fengying Fan, Xin Chao, Jin Liu, Chuanmin Pan, Qunxing Sun, Huawei Zhang, Xiaofei Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings |
title | Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings |
title_full | Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings |
title_fullStr | Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings |
title_full_unstemmed | Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings |
title_short | Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings |
title_sort | immune-related mirna-mrna regulation network in the livers of dhav-3-infected ducklings |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001231/ https://www.ncbi.nlm.nih.gov/pubmed/32019511 http://dx.doi.org/10.1186/s12864-020-6539-7 |
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