Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

BACKGROUND: Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings remains poorly understood. To study the genetic regulatory network for miRNA-mRNA and the signaling pathways involve...

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Autores principales: Wu, Fengyao, Lu, Fengying, Fan, Xin, Chao, Jin, Liu, Chuanmin, Pan, Qunxing, Sun, Huawei, Zhang, Xiaofei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001231/
https://www.ncbi.nlm.nih.gov/pubmed/32019511
http://dx.doi.org/10.1186/s12864-020-6539-7
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author Wu, Fengyao
Lu, Fengying
Fan, Xin
Chao, Jin
Liu, Chuanmin
Pan, Qunxing
Sun, Huawei
Zhang, Xiaofei
author_facet Wu, Fengyao
Lu, Fengying
Fan, Xin
Chao, Jin
Liu, Chuanmin
Pan, Qunxing
Sun, Huawei
Zhang, Xiaofei
author_sort Wu, Fengyao
collection PubMed
description BACKGROUND: Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings remains poorly understood. To study the genetic regulatory network for miRNA-mRNA and the signaling pathways involved in DHAV-3 infection in ducklings, we conducted global miRNA and mRNA expression profiling of duckling liver tissues infected with lethal DHAV-3 by high-throughput sequencing. RESULTS: We found 156 differentially expressed miRNAs (DEMs) and 7717 differentially expressed genes (DEGs) in livers of mock-infected and DHAV-3-infected duckling. A total of 19,606 miRNA-mRNA pairs with negatively correlated expression patterns were identified in miRNA-mRNA networks constructed on the basis of these DEMs and DEGs. Moreover, immune-related pathways, including the cytokine-cytokine receptor interaction, apoptosis, Toll-like receptor, Jak-STAT, and RIG-I-like receptor signaling pathway, were significantly enriched through analyzing functions of mRNAs in the network in response to DHAV-3 infection. Furthermore, apl-miR-32-5p, apl-miR-125-5p, apl-miR-128-3p, apl-miR-460-5p, and novel-m0012-3p were identified as potential regulators in the immune-related signaling pathways during DHAV-3 infection. And some host miRNAs were predicted to target the DHAV-3 genome. CONCLUSIONS: This is the first integrated analysis of miRNA and mRNA in DHAV-3-infected ducklings. The results indicated the important roles of miRNAs in regulating immune response genes and revealed the immune related miRNA-mRNA regulation network in the DHAV-3-infected duckling liver. These findings increase our knowledge of the roles of miRNAs and their target genes in DHAV-3 replication and pathogenesis. They also aid in the understanding of host-virus interactions.
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spelling pubmed-70012312020-02-10 Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings Wu, Fengyao Lu, Fengying Fan, Xin Chao, Jin Liu, Chuanmin Pan, Qunxing Sun, Huawei Zhang, Xiaofei BMC Genomics Research Article BACKGROUND: Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings remains poorly understood. To study the genetic regulatory network for miRNA-mRNA and the signaling pathways involved in DHAV-3 infection in ducklings, we conducted global miRNA and mRNA expression profiling of duckling liver tissues infected with lethal DHAV-3 by high-throughput sequencing. RESULTS: We found 156 differentially expressed miRNAs (DEMs) and 7717 differentially expressed genes (DEGs) in livers of mock-infected and DHAV-3-infected duckling. A total of 19,606 miRNA-mRNA pairs with negatively correlated expression patterns were identified in miRNA-mRNA networks constructed on the basis of these DEMs and DEGs. Moreover, immune-related pathways, including the cytokine-cytokine receptor interaction, apoptosis, Toll-like receptor, Jak-STAT, and RIG-I-like receptor signaling pathway, were significantly enriched through analyzing functions of mRNAs in the network in response to DHAV-3 infection. Furthermore, apl-miR-32-5p, apl-miR-125-5p, apl-miR-128-3p, apl-miR-460-5p, and novel-m0012-3p were identified as potential regulators in the immune-related signaling pathways during DHAV-3 infection. And some host miRNAs were predicted to target the DHAV-3 genome. CONCLUSIONS: This is the first integrated analysis of miRNA and mRNA in DHAV-3-infected ducklings. The results indicated the important roles of miRNAs in regulating immune response genes and revealed the immune related miRNA-mRNA regulation network in the DHAV-3-infected duckling liver. These findings increase our knowledge of the roles of miRNAs and their target genes in DHAV-3 replication and pathogenesis. They also aid in the understanding of host-virus interactions. BioMed Central 2020-02-04 /pmc/articles/PMC7001231/ /pubmed/32019511 http://dx.doi.org/10.1186/s12864-020-6539-7 Text en © The Author(s). 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wu, Fengyao
Lu, Fengying
Fan, Xin
Chao, Jin
Liu, Chuanmin
Pan, Qunxing
Sun, Huawei
Zhang, Xiaofei
Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings
title Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings
title_full Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings
title_fullStr Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings
title_full_unstemmed Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings
title_short Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings
title_sort immune-related mirna-mrna regulation network in the livers of dhav-3-infected ducklings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001231/
https://www.ncbi.nlm.nih.gov/pubmed/32019511
http://dx.doi.org/10.1186/s12864-020-6539-7
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