LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites

BACKGROUND: Ligation-mediated PCR protocols have diverse uses including the identification of integration sites of insertional mutagens, integrating vectors and naturally occurring mobile genetic elements. For approaches that employ NGS sequencing, the relative abundance of integrations within a com...

Descripción completa

Detalles Bibliográficos
Autores principales: Dawes, Joanna C., Webster, Philip, Iadarola, Barbara, Garcia-Diaz, Claudia, Dore, Marian, Bolt, Bruce J., Dewchand, Hamlata, Dharmalingam, Gopuraja, McLatchie, Alex P., Kaczor, Jakub, Caceres, Juan J., Paccanaro, Alberto, Game, Laurence, Parrinello, Simona, Uren, Anthony G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001329/
https://www.ncbi.nlm.nih.gov/pubmed/32042315
http://dx.doi.org/10.1186/s13100-020-0201-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7001329/
https://www.ncbi.nlm.nih.gov/pubmed/32042315
http://dx.doi.org/10.1186/s13100-020-0201-4

Ejemplares similares