Improving sensitivity of single tube nested PCR to detect fastidious microorganisms

Single Tube Nested PCR (ST-nPCR) is of value to clinical laboratories with limited settings for the detection of fastidious microorganisms. The detection sensitivity of ST-nPCR is dependent on ensuring minimal leftovers of outer primers during the second round of the reaction. In this work, we inves...

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Detalles Bibliográficos
Autores principales: Shatleh-Rantisi, Diala, Tamimi, Asmaa, Ashhab, Yaqoub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002839/
https://www.ncbi.nlm.nih.gov/pubmed/32042975
http://dx.doi.org/10.1016/j.heliyon.2020.e03246

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002839/
https://www.ncbi.nlm.nih.gov/pubmed/32042975
http://dx.doi.org/10.1016/j.heliyon.2020.e03246

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