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Improving sensitivity of single tube nested PCR to detect fastidious microorganisms
Single Tube Nested PCR (ST-nPCR) is of value to clinical laboratories with limited settings for the detection of fastidious microorganisms. The detection sensitivity of ST-nPCR is dependent on ensuring minimal leftovers of outer primers during the second round of the reaction. In this work, we inves...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7002839/ https://www.ncbi.nlm.nih.gov/pubmed/32042975 http://dx.doi.org/10.1016/j.heliyon.2020.e03246 |