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SapTrap Assembly of Caenorhabditis elegans MosSCI Transgene Vectors

The Mos1-mediated Single-Copy Insertion (MosSCI) method is widely used to establish stable Caenorhabditis elegans transgenic strains. Cloning MosSCI targeting plasmids can be cumbersome because it requires assembling multiple genetic elements including a promoter, a 3′UTR and gene fragments. Recentl...

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Detalles Bibliográficos
Autores principales: Fan, Xintao, De Henau, Sasha, Feinstein, Julia, Miller, Stephanie I., Han, Bingjie, Frøkjær-Jensen, Christian, Griffin, Erik E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003106/
https://www.ncbi.nlm.nih.gov/pubmed/31848219
http://dx.doi.org/10.1534/g3.119.400822