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SapTrap Assembly of Caenorhabditis elegans MosSCI Transgene Vectors
The Mos1-mediated Single-Copy Insertion (MosSCI) method is widely used to establish stable Caenorhabditis elegans transgenic strains. Cloning MosSCI targeting plasmids can be cumbersome because it requires assembling multiple genetic elements including a promoter, a 3′UTR and gene fragments. Recentl...
Autores principales: | Fan, Xintao, De Henau, Sasha, Feinstein, Julia, Miller, Stephanie I., Han, Bingjie, Frøkjær-Jensen, Christian, Griffin, Erik E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7003106/ https://www.ncbi.nlm.nih.gov/pubmed/31848219 http://dx.doi.org/10.1534/g3.119.400822 |
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