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Two-phase wash to solve the ubiquitous contaminant-carryover problem in commercial nucleic-acid extraction kits

The success of fundamental and applied nucleic acid (NA) research depends on NA purity, but obtaining pure NAs from raw, unprocessed samples is challenging. Purification using solid-phase NA extractions utilizes sequential additions of lysis and wash buffers followed by elution. The resulting eluent...

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Detalles Bibliográficos
Autores principales: Jue, Erik, Witters, Daan, Ismagilov, Rustem F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7004994/
https://www.ncbi.nlm.nih.gov/pubmed/32029846
http://dx.doi.org/10.1038/s41598-020-58586-3