Genetic screens in isogenic mammalian cell lines without single cell cloning

Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools for understanding gene function. Generating such pairs of mammalian cells, however, is labor-intensive, time-consuming, and, in some cell types, essentially impossible. Here, we present an approach to cre...

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Detalles Bibliográficos
Autores principales: DeWeirdt, Peter C., Sangree, Annabel K., Hanna, Ruth E., Sanson, Kendall R., Hegde, Mudra, Strand, Christine, Persky, Nicole S., Doench, John G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005275/
https://www.ncbi.nlm.nih.gov/pubmed/32029722
http://dx.doi.org/10.1038/s41467-020-14620-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005275/
https://www.ncbi.nlm.nih.gov/pubmed/32029722
http://dx.doi.org/10.1038/s41467-020-14620-6

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