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Functional dynamics of a single tryptophan residue in a BLUF protein revealed by fluorescence spectroscopy
Blue Light Using Flavin (BLUF) domains are increasingly being adopted for use in optogenetic constructs. Despite this, much remains to be resolved on the mechanism of their activation. The advent of unnatural amino acid mutagenesis opens up a new toolbox for the study of protein structural dynamics....
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7005313/ https://www.ncbi.nlm.nih.gov/pubmed/32029866 http://dx.doi.org/10.1038/s41598-020-59073-5 |