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Small-molecule targeted recruitment of a nuclease to cleave an oncogenic RNA in a mouse model of metastatic cancer
As the area of small molecules interacting with RNA advances, general routes to provide bioactive compounds are needed as ligands can bind RNA avidly to sites that will not affect function. Small-molecule targeted RNA degradation will thus provide a general route to affect RNA biology. A non–oligonu...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007575/ https://www.ncbi.nlm.nih.gov/pubmed/31964809 http://dx.doi.org/10.1073/pnas.1914286117 |
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author | Costales, Matthew G. Aikawa, Haruo Li, Yue Childs-Disney, Jessica L. Abegg, Daniel Hoch, Dominic G. Pradeep Velagapudi, Sai Nakai, Yoshio Khan, Tanya Wang, Kye Won Yildirim, Ilyas Adibekian, Alexander Wang, Eric T. Disney, Matthew D. |
author_facet | Costales, Matthew G. Aikawa, Haruo Li, Yue Childs-Disney, Jessica L. Abegg, Daniel Hoch, Dominic G. Pradeep Velagapudi, Sai Nakai, Yoshio Khan, Tanya Wang, Kye Won Yildirim, Ilyas Adibekian, Alexander Wang, Eric T. Disney, Matthew D. |
author_sort | Costales, Matthew G. |
collection | PubMed |
description | As the area of small molecules interacting with RNA advances, general routes to provide bioactive compounds are needed as ligands can bind RNA avidly to sites that will not affect function. Small-molecule targeted RNA degradation will thus provide a general route to affect RNA biology. A non–oligonucleotide-containing compound was designed from sequence to target the precursor to oncogenic microRNA-21 (pre–miR-21) for enzymatic destruction with selectivity that can exceed that for protein-targeted medicines. The compound specifically binds the target and contains a heterocycle that recruits and activates a ribonuclease to pre–miR-21 to substoichiometrically effect its cleavage and subsequently impede metastasis of breast cancer to lung in a mouse model. Transcriptomic and proteomic analyses demonstrate that the compound is potent and selective, specifically modulating oncogenic pathways. Thus, small molecules can be designed from sequence to have all of the functional repertoire of oligonucleotides, including inducing enzymatic degradation, and to selectively and potently modulate RNA function in vivo. |
format | Online Article Text |
id | pubmed-7007575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-70075752020-02-18 Small-molecule targeted recruitment of a nuclease to cleave an oncogenic RNA in a mouse model of metastatic cancer Costales, Matthew G. Aikawa, Haruo Li, Yue Childs-Disney, Jessica L. Abegg, Daniel Hoch, Dominic G. Pradeep Velagapudi, Sai Nakai, Yoshio Khan, Tanya Wang, Kye Won Yildirim, Ilyas Adibekian, Alexander Wang, Eric T. Disney, Matthew D. Proc Natl Acad Sci U S A Biological Sciences As the area of small molecules interacting with RNA advances, general routes to provide bioactive compounds are needed as ligands can bind RNA avidly to sites that will not affect function. Small-molecule targeted RNA degradation will thus provide a general route to affect RNA biology. A non–oligonucleotide-containing compound was designed from sequence to target the precursor to oncogenic microRNA-21 (pre–miR-21) for enzymatic destruction with selectivity that can exceed that for protein-targeted medicines. The compound specifically binds the target and contains a heterocycle that recruits and activates a ribonuclease to pre–miR-21 to substoichiometrically effect its cleavage and subsequently impede metastasis of breast cancer to lung in a mouse model. Transcriptomic and proteomic analyses demonstrate that the compound is potent and selective, specifically modulating oncogenic pathways. Thus, small molecules can be designed from sequence to have all of the functional repertoire of oligonucleotides, including inducing enzymatic degradation, and to selectively and potently modulate RNA function in vivo. National Academy of Sciences 2020-02-04 2020-01-21 /pmc/articles/PMC7007575/ /pubmed/31964809 http://dx.doi.org/10.1073/pnas.1914286117 Text en Copyright © 2020 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Costales, Matthew G. Aikawa, Haruo Li, Yue Childs-Disney, Jessica L. Abegg, Daniel Hoch, Dominic G. Pradeep Velagapudi, Sai Nakai, Yoshio Khan, Tanya Wang, Kye Won Yildirim, Ilyas Adibekian, Alexander Wang, Eric T. Disney, Matthew D. Small-molecule targeted recruitment of a nuclease to cleave an oncogenic RNA in a mouse model of metastatic cancer |
title | Small-molecule targeted recruitment of a nuclease to cleave an oncogenic RNA in a mouse model of metastatic cancer |
title_full | Small-molecule targeted recruitment of a nuclease to cleave an oncogenic RNA in a mouse model of metastatic cancer |
title_fullStr | Small-molecule targeted recruitment of a nuclease to cleave an oncogenic RNA in a mouse model of metastatic cancer |
title_full_unstemmed | Small-molecule targeted recruitment of a nuclease to cleave an oncogenic RNA in a mouse model of metastatic cancer |
title_short | Small-molecule targeted recruitment of a nuclease to cleave an oncogenic RNA in a mouse model of metastatic cancer |
title_sort | small-molecule targeted recruitment of a nuclease to cleave an oncogenic rna in a mouse model of metastatic cancer |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7007575/ https://www.ncbi.nlm.nih.gov/pubmed/31964809 http://dx.doi.org/10.1073/pnas.1914286117 |
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