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Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging
Two-photon excitation microscopy is one of the key techniques used to observe three-dimensional (3-D) structures in biological samples. We utilized a visible-wavelength laser beam for two-photon excitation in a multifocus confocal scanning system to improve the spatial resolution and image contrast...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society of Photo-Optical Instrumentation Engineers
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7008499/ https://www.ncbi.nlm.nih.gov/pubmed/31691550 http://dx.doi.org/10.1117/1.JBO.25.1.014502 |