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An optimized workflow for single-cell transcriptomics and repertoire profiling of purified lymphocytes from clinical samples
Establishing clinically relevant single-cell (SC) transcriptomic workflows from cryopreserved tissue is essential to move this emerging immune monitoring technology from the bench to the bedside. Improper sample preparation leads to detrimental cascades, resulting in loss of precious time, money and...
Autores principales: | Hanamsagar, Richa, Reizis, Timothy, Chamberlain, Mathew, Marcus, Robert, Nestle, Frank O., de Rinaldis, Emanuele, Savova, Virginia |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010687/ https://www.ncbi.nlm.nih.gov/pubmed/32042039 http://dx.doi.org/10.1038/s41598-020-58939-y |
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