Specificity profiling of CRISPR system reveals greatly enhanced off-target gene editing

To explore the editing specificity of CRISPR/Cpf1 system, effects of target mutation were systematically examined using a reporter activation assay, with a set of single-nucleotide mutated target site. Consistent with our previous study performed with CRISPR/Cas9, a “core” sequence region that is hi...

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Detalles Bibliográficos
Autores principales: Wang, Yao, Wang, Mingrui, Zheng, Ting, Hou, Yingzi, Zhang, Pingjing, Tang, Tao, Wei, Jing, Du, Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010781/
https://www.ncbi.nlm.nih.gov/pubmed/32042045
http://dx.doi.org/10.1038/s41598-020-58627-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010781/
https://www.ncbi.nlm.nih.gov/pubmed/32042045
http://dx.doi.org/10.1038/s41598-020-58627-x

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