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REP-X: An Evolution-guided Strategy for the Rational Design of Cysteine-less Protein Variants

Site-specific labeling of proteins is often a prerequisite for biophysical and biochemical characterization. Chemical modification of a unique cysteine residue is among the most facile methods for site-specific labeling of proteins. However, many proteins have multiple reactive cysteines, which must...

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Detalles Bibliográficos
Autores principales: Dalton, Kevin, Lopez, Tom, Pande, Vijay, Frydman, Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010797/
https://www.ncbi.nlm.nih.gov/pubmed/32042106
http://dx.doi.org/10.1038/s41598-020-58794-x