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REP-X: An Evolution-guided Strategy for the Rational Design of Cysteine-less Protein Variants
Site-specific labeling of proteins is often a prerequisite for biophysical and biochemical characterization. Chemical modification of a unique cysteine residue is among the most facile methods for site-specific labeling of proteins. However, many proteins have multiple reactive cysteines, which must...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7010797/ https://www.ncbi.nlm.nih.gov/pubmed/32042106 http://dx.doi.org/10.1038/s41598-020-58794-x |