CRISPR-Cas12a Possesses Unconventional DNase Activity that Can Be Inactivated by Synthetic Oligonucleotides

CRISPR-Cas12a (CRISPR-Cpf1) was reported to have multiple types of cleavage activities. Without the assistance of CRISPR RNA (crRNA), we investigated DNase activity and substrate specificity of Cas12a orthologs in the presence of diverse divalent metal ions. Cas12a from different species are capable...

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Detalles Bibliográficos
Autores principales: Li, Bin, Yan, Jingyue, Zhang, Youxi, Li, Wenqing, Zeng, Chunxi, Zhao, Weiyu, Hou, Xucheng, Zhang, Chengxiang, Dong, Yizhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015830/
https://www.ncbi.nlm.nih.gov/pubmed/32045875
http://dx.doi.org/10.1016/j.omtn.2019.12.038

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015830/
https://www.ncbi.nlm.nih.gov/pubmed/32045875
http://dx.doi.org/10.1016/j.omtn.2019.12.038

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