Enhancing Terminal Deoxynucleotidyl Transferase Activity on Substrates with 3′ Terminal Structures for Enzymatic De Novo DNA Synthesis

Enzymatic oligonucleotide synthesis methods based on the template-independent polymerase terminal deoxynucleotidyl transferase (TdT) promise to enable the de novo synthesis of long oligonucleotides under mild, aqueous conditions. Intermediates with a 3′ terminal structure (hairpins) will inevitably...

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Detalles Bibliográficos
Autores principales: Barthel, Sebastian, Palluk, Sebastian, Hillson, Nathan J., Keasling, Jay D., Arlow, Daniel H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016565/
https://www.ncbi.nlm.nih.gov/pubmed/31963235
http://dx.doi.org/10.3390/genes11010102

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016565/
https://www.ncbi.nlm.nih.gov/pubmed/31963235
http://dx.doi.org/10.3390/genes11010102

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