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Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy

PDCD4 (programmed cell death 4) is a tumor suppressor that plays a crucial role in multiple cellular functions, such as the control of protein synthesis and transcriptional control of some genes, the inhibition of cancer invasion and metastasis. The expression of this protein is controlled by synthe...

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Autores principales: Manirujjaman, M., Ozaki, Iwata, Murata, Yuzo, Guo, Jing, Xia, Jinghe, Nishioka, Kenichi, Perveen, Rasheda, Takahashi, Hirokazu, Anzai, Keizo, Matsuhashi, Sachiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016974/
https://www.ncbi.nlm.nih.gov/pubmed/31952347
http://dx.doi.org/10.3390/cells9010218
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author Manirujjaman, M.
Ozaki, Iwata
Murata, Yuzo
Guo, Jing
Xia, Jinghe
Nishioka, Kenichi
Perveen, Rasheda
Takahashi, Hirokazu
Anzai, Keizo
Matsuhashi, Sachiko
author_facet Manirujjaman, M.
Ozaki, Iwata
Murata, Yuzo
Guo, Jing
Xia, Jinghe
Nishioka, Kenichi
Perveen, Rasheda
Takahashi, Hirokazu
Anzai, Keizo
Matsuhashi, Sachiko
author_sort Manirujjaman, M.
collection PubMed
description PDCD4 (programmed cell death 4) is a tumor suppressor that plays a crucial role in multiple cellular functions, such as the control of protein synthesis and transcriptional control of some genes, the inhibition of cancer invasion and metastasis. The expression of this protein is controlled by synthesis, such as via transcription and translation, and degradation by the ubiquitin-proteasome system. The mitogens, known as tumor promotors, EGF (epidermal growth factor) and TPA (12-O-tetradecanoylphorbol-13-acetate) stimulate the degradation of PDCD4 protein. However, the whole picture of PDCD4 degradation mechanisms is still unclear, we therefore investigated the relationship between PDCD4 and autophagy. The proteasome inhibitor MG132 and the autophagy inhibitor bafilomycin A1 were found to upregulate the PDCD4 levels. PDCD4 protein levels increased synergistically in the presence of both inhibitors. Knockdown of p62/SQSTM1 (sequestosome-1), a polyubiquitin binding partner, also upregulated the PDCD4 levels. P62 and LC3 (microtubule-associated protein 1A/1B-light chain 3)-II were co-immunoprecipitated by an anti-PDCD4 antibody. Colocalization particles of PDCD4, p62 and the autophagosome marker LC3 were observed and the colocalization areas increased in the presence of autophagy and/or proteasome inhibitor(s) in Huh7 cells. In ATG (autophagy related) 5-deficient Huh7 cells in which autophagy was impaired, the PDCD4 levels were increased at the basal levels and upregulated in the presence of autophagy inhibitors. Based on the above findings, we concluded that after phosphorylation in the degron and ubiquitination, PDCD4 is degraded by both the proteasome and autophagy systems.
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spelling pubmed-70169742020-02-28 Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy Manirujjaman, M. Ozaki, Iwata Murata, Yuzo Guo, Jing Xia, Jinghe Nishioka, Kenichi Perveen, Rasheda Takahashi, Hirokazu Anzai, Keizo Matsuhashi, Sachiko Cells Article PDCD4 (programmed cell death 4) is a tumor suppressor that plays a crucial role in multiple cellular functions, such as the control of protein synthesis and transcriptional control of some genes, the inhibition of cancer invasion and metastasis. The expression of this protein is controlled by synthesis, such as via transcription and translation, and degradation by the ubiquitin-proteasome system. The mitogens, known as tumor promotors, EGF (epidermal growth factor) and TPA (12-O-tetradecanoylphorbol-13-acetate) stimulate the degradation of PDCD4 protein. However, the whole picture of PDCD4 degradation mechanisms is still unclear, we therefore investigated the relationship between PDCD4 and autophagy. The proteasome inhibitor MG132 and the autophagy inhibitor bafilomycin A1 were found to upregulate the PDCD4 levels. PDCD4 protein levels increased synergistically in the presence of both inhibitors. Knockdown of p62/SQSTM1 (sequestosome-1), a polyubiquitin binding partner, also upregulated the PDCD4 levels. P62 and LC3 (microtubule-associated protein 1A/1B-light chain 3)-II were co-immunoprecipitated by an anti-PDCD4 antibody. Colocalization particles of PDCD4, p62 and the autophagosome marker LC3 were observed and the colocalization areas increased in the presence of autophagy and/or proteasome inhibitor(s) in Huh7 cells. In ATG (autophagy related) 5-deficient Huh7 cells in which autophagy was impaired, the PDCD4 levels were increased at the basal levels and upregulated in the presence of autophagy inhibitors. Based on the above findings, we concluded that after phosphorylation in the degron and ubiquitination, PDCD4 is degraded by both the proteasome and autophagy systems. MDPI 2020-01-15 /pmc/articles/PMC7016974/ /pubmed/31952347 http://dx.doi.org/10.3390/cells9010218 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Manirujjaman, M.
Ozaki, Iwata
Murata, Yuzo
Guo, Jing
Xia, Jinghe
Nishioka, Kenichi
Perveen, Rasheda
Takahashi, Hirokazu
Anzai, Keizo
Matsuhashi, Sachiko
Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy
title Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy
title_full Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy
title_fullStr Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy
title_full_unstemmed Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy
title_short Degradation of the Tumor Suppressor PDCD4 Is Impaired by the Suppression of p62/SQSTM1 and Autophagy
title_sort degradation of the tumor suppressor pdcd4 is impaired by the suppression of p62/sqstm1 and autophagy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7016974/
https://www.ncbi.nlm.nih.gov/pubmed/31952347
http://dx.doi.org/10.3390/cells9010218
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