Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system

Ganoderma lucidum is an important medicinal mushroom in traditional Chinese medicine. However, the lack of adequate genetic tools has hindered molecular genetic research in and the genetic modification of this species. Here, we report that the presence of an intron is necessary for the efficient exp...

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Detalles Bibliográficos
Autores principales: Liu, Ke, Sun, Bin, You, Hao, Tu, Jun‐Liang, Yu, Xuya, Zhao, Peng, Xu, Jun‐Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7017817/
https://www.ncbi.nlm.nih.gov/pubmed/31958883
http://dx.doi.org/10.1111/1751-7915.13534

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7017817/
https://www.ncbi.nlm.nih.gov/pubmed/31958883
http://dx.doi.org/10.1111/1751-7915.13534

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