Toward Standards in Clinical Microbiota Studies: Comparison of Three DNA Extraction Methods and Two Bioinformatic Pipelines

When studying the microbiome using next-generation sequencing, the DNA extraction method, sequencing procedures, and bioinformatic processing are crucial to obtain reliable data. Method choice has been demonstrated to strongly affect the final biological interpretation. We assessed the performance o...

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Detalles Bibliográficos
Autores principales: Ducarmon, Q. R., Hornung, B. V. H., Geelen, A. R., Kuijper, E. J., Zwittink, R. D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018525/
https://www.ncbi.nlm.nih.gov/pubmed/32047058
http://dx.doi.org/10.1128/mSystems.00547-19

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018525/
https://www.ncbi.nlm.nih.gov/pubmed/32047058
http://dx.doi.org/10.1128/mSystems.00547-19

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