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Toward Standards in Clinical Microbiota Studies: Comparison of Three DNA Extraction Methods and Two Bioinformatic Pipelines
When studying the microbiome using next-generation sequencing, the DNA extraction method, sequencing procedures, and bioinformatic processing are crucial to obtain reliable data. Method choice has been demonstrated to strongly affect the final biological interpretation. We assessed the performance o...
Autores principales: | Ducarmon, Q. R., Hornung, B. V. H., Geelen, A. R., Kuijper, E. J., Zwittink, R. D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7018525/ https://www.ncbi.nlm.nih.gov/pubmed/32047058 http://dx.doi.org/10.1128/mSystems.00547-19 |
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