Cargando…

TREX1 531C>T Polymorphism is Associated with High Proviral Load Levels in HTLV-1-Infected Persons

Human T-lymphotropic virus type 1 (HTLV-1) deregulates the immune system and cell cycle, resulting in loss of immune tolerance and disease, including HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Three prime repair exonuclease 1 (TREX1) maintains innate immune tolerance of the...

Descripción completa

Detalles Bibliográficos
Autores principales: Silva, Denis de Castro, Amoras, Ednelza da Silva Graça, Moura, Tuane Carolina Ferreira, Lopes, Felipe Teixeira, Gomes, Samara Tatielle Monteiro, da Costa, Carlos A., Sousa, Maísa Silva, Ishak, Ricardo, Vallinoto, Antonio Carlos Rosário, Queiroz, Maria Alice Freitas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019804/
https://www.ncbi.nlm.nih.gov/pubmed/31861565
http://dx.doi.org/10.3390/v12010007
_version_ 1783497604197253120
author Silva, Denis de Castro
Amoras, Ednelza da Silva Graça
Moura, Tuane Carolina Ferreira
Lopes, Felipe Teixeira
Gomes, Samara Tatielle Monteiro
da Costa, Carlos A.
Sousa, Maísa Silva
Ishak, Ricardo
Vallinoto, Antonio Carlos Rosário
Queiroz, Maria Alice Freitas
author_facet Silva, Denis de Castro
Amoras, Ednelza da Silva Graça
Moura, Tuane Carolina Ferreira
Lopes, Felipe Teixeira
Gomes, Samara Tatielle Monteiro
da Costa, Carlos A.
Sousa, Maísa Silva
Ishak, Ricardo
Vallinoto, Antonio Carlos Rosário
Queiroz, Maria Alice Freitas
author_sort Silva, Denis de Castro
collection PubMed
description Human T-lymphotropic virus type 1 (HTLV-1) deregulates the immune system and cell cycle, resulting in loss of immune tolerance and disease, including HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Three prime repair exonuclease 1 (TREX1) maintains innate immune tolerance of the host and host-cell permissiveness to retroviral infections. TREX1 polymorphisms may influence the course of infection and autoimmune manifestations. The influence of TREX1 531C/T polymorphism was investigated in HTLV-1 infection and development of symptoms among 151 persons infected with HTLV-1 (32 HAM/TSP, 19 rheumatologic manifestations, two dermatitis, five more than one diagnosis, two probable HAM/TSP, and 91 asymptomatic individuals) and 100 uninfected persons in the control group. Polymorphism genotyping and proviral load quantification were performed by real-time polymerase chain reaction (PCR) and antinuclear antibodies (ANAs) were screened by an indirect immunofluorescence assay. No statistically significant difference was found in polymorphism genotype and allele frequencies between the infected and control groups. HAM/TSP patients showed higher frequency of TT genotype than asymptomatic persons (p = 0.0339). Proviral load was significantly higher among individuals with CT/TT genotypes and CC genotype carriers had lower proviral load and higher levels of proinflammatory cytokines. ANAs were present only in the HAM/TSP group. TREX1 531C>T polymorphism seems to be associated with TREX-1 regulation and HTLV-1 infection.
format Online
Article
Text
id pubmed-7019804
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70198042020-03-09 TREX1 531C>T Polymorphism is Associated with High Proviral Load Levels in HTLV-1-Infected Persons Silva, Denis de Castro Amoras, Ednelza da Silva Graça Moura, Tuane Carolina Ferreira Lopes, Felipe Teixeira Gomes, Samara Tatielle Monteiro da Costa, Carlos A. Sousa, Maísa Silva Ishak, Ricardo Vallinoto, Antonio Carlos Rosário Queiroz, Maria Alice Freitas Viruses Article Human T-lymphotropic virus type 1 (HTLV-1) deregulates the immune system and cell cycle, resulting in loss of immune tolerance and disease, including HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Three prime repair exonuclease 1 (TREX1) maintains innate immune tolerance of the host and host-cell permissiveness to retroviral infections. TREX1 polymorphisms may influence the course of infection and autoimmune manifestations. The influence of TREX1 531C/T polymorphism was investigated in HTLV-1 infection and development of symptoms among 151 persons infected with HTLV-1 (32 HAM/TSP, 19 rheumatologic manifestations, two dermatitis, five more than one diagnosis, two probable HAM/TSP, and 91 asymptomatic individuals) and 100 uninfected persons in the control group. Polymorphism genotyping and proviral load quantification were performed by real-time polymerase chain reaction (PCR) and antinuclear antibodies (ANAs) were screened by an indirect immunofluorescence assay. No statistically significant difference was found in polymorphism genotype and allele frequencies between the infected and control groups. HAM/TSP patients showed higher frequency of TT genotype than asymptomatic persons (p = 0.0339). Proviral load was significantly higher among individuals with CT/TT genotypes and CC genotype carriers had lower proviral load and higher levels of proinflammatory cytokines. ANAs were present only in the HAM/TSP group. TREX1 531C>T polymorphism seems to be associated with TREX-1 regulation and HTLV-1 infection. MDPI 2019-12-19 /pmc/articles/PMC7019804/ /pubmed/31861565 http://dx.doi.org/10.3390/v12010007 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Silva, Denis de Castro
Amoras, Ednelza da Silva Graça
Moura, Tuane Carolina Ferreira
Lopes, Felipe Teixeira
Gomes, Samara Tatielle Monteiro
da Costa, Carlos A.
Sousa, Maísa Silva
Ishak, Ricardo
Vallinoto, Antonio Carlos Rosário
Queiroz, Maria Alice Freitas
TREX1 531C>T Polymorphism is Associated with High Proviral Load Levels in HTLV-1-Infected Persons
title TREX1 531C>T Polymorphism is Associated with High Proviral Load Levels in HTLV-1-Infected Persons
title_full TREX1 531C>T Polymorphism is Associated with High Proviral Load Levels in HTLV-1-Infected Persons
title_fullStr TREX1 531C>T Polymorphism is Associated with High Proviral Load Levels in HTLV-1-Infected Persons
title_full_unstemmed TREX1 531C>T Polymorphism is Associated with High Proviral Load Levels in HTLV-1-Infected Persons
title_short TREX1 531C>T Polymorphism is Associated with High Proviral Load Levels in HTLV-1-Infected Persons
title_sort trex1 531c>t polymorphism is associated with high proviral load levels in htlv-1-infected persons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7019804/
https://www.ncbi.nlm.nih.gov/pubmed/31861565
http://dx.doi.org/10.3390/v12010007
work_keys_str_mv AT silvadenisdecastro trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons
AT amorasednelzadasilvagraca trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons
AT mouratuanecarolinaferreira trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons
AT lopesfelipeteixeira trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons
AT gomessamaratatiellemonteiro trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons
AT dacostacarlosa trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons
AT sousamaisasilva trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons
AT ishakricardo trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons
AT vallinotoantoniocarlosrosario trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons
AT queirozmariaalicefreitas trex1531ctpolymorphismisassociatedwithhighproviralloadlevelsinhtlv1infectedpersons