RNA sequencing by direct tagmentation of RNA/DNA hybrids

Transcriptome profiling by RNA sequencing (RNA-seq) has been widely used to characterize cellular status, but it relies on second-strand complementary DNA (cDNA) synthesis to generate initial material for library preparation. Here we use bacterial transposase Tn5, which has been increasingly used in...

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Detalles Bibliográficos
Autores principales: Di, Lin, Fu, Yusi, Sun, Yue, Li, Jie, Liu, Lu, Yao, Jiacheng, Wang, Guanbo, Wu, Yalei, Lao, Kaiqin, Lee, Raymond W., Zheng, Genhua, Xu, Jun, Oh, Juntaek, Wang, Dong, Xie, X. Sunney, Huang, Yanyi, Wang, Jianbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2020
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022195/
https://www.ncbi.nlm.nih.gov/pubmed/31988135
http://dx.doi.org/10.1073/pnas.1919800117

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022195/
https://www.ncbi.nlm.nih.gov/pubmed/31988135
http://dx.doi.org/10.1073/pnas.1919800117

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