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Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.)

Rice (Oryza sativa L.) is the staple food of more than half of the world’s population. The main factors affecting the quality of rice include grain length, texture, stickiness, flavor, and aroma. Pesticides are intended for the protection of plant products from weeds, fungi, or insects. However, pes...

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Autores principales: Melo, Maria Graça, Carqueijo, Ana, Freitas, Andreia, Barbosa, Jorge, Silva, Ana Sanches
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022397/
https://www.ncbi.nlm.nih.gov/pubmed/31878165
http://dx.doi.org/10.3390/foods9010018
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author Melo, Maria Graça
Carqueijo, Ana
Freitas, Andreia
Barbosa, Jorge
Silva, Ana Sanches
author_facet Melo, Maria Graça
Carqueijo, Ana
Freitas, Andreia
Barbosa, Jorge
Silva, Ana Sanches
author_sort Melo, Maria Graça
collection PubMed
description Rice (Oryza sativa L.) is the staple food of more than half of the world’s population. The main factors affecting the quality of rice include grain length, texture, stickiness, flavor, and aroma. Pesticides are intended for the protection of plant products from weeds, fungi, or insects. However, pesticides also result in negative effects such as environment disturbances, pest resistance and toxicity to both users and food consumers. The aim of this study was to conduct validation experiments of a method for the determination of multi-pesticides in rice, a model food of other cereals. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was used for the extraction of pesticide residues from rice followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with a triple quadrupole instrument using electrospray ionization. The analytical method has chromatography-tandem according to SANTE/11813/2017. The limit of quantification was 5 μg/kg. Recoveries for the 155 analyzed pesticides ranged between 77.1% for pirimiphos-ethyl and 111.5% for flutriafol and they were determined at 3 spiking levels. The proposed method was demonstrated to be quick, simple, precise, and accurate and allowed for evaluating the compliance of cereals samples with legislated maximum residue levels of pesticides in the European Union.
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spelling pubmed-70223972020-03-09 Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.) Melo, Maria Graça Carqueijo, Ana Freitas, Andreia Barbosa, Jorge Silva, Ana Sanches Foods Article Rice (Oryza sativa L.) is the staple food of more than half of the world’s population. The main factors affecting the quality of rice include grain length, texture, stickiness, flavor, and aroma. Pesticides are intended for the protection of plant products from weeds, fungi, or insects. However, pesticides also result in negative effects such as environment disturbances, pest resistance and toxicity to both users and food consumers. The aim of this study was to conduct validation experiments of a method for the determination of multi-pesticides in rice, a model food of other cereals. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was used for the extraction of pesticide residues from rice followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with a triple quadrupole instrument using electrospray ionization. The analytical method has chromatography-tandem according to SANTE/11813/2017. The limit of quantification was 5 μg/kg. Recoveries for the 155 analyzed pesticides ranged between 77.1% for pirimiphos-ethyl and 111.5% for flutriafol and they were determined at 3 spiking levels. The proposed method was demonstrated to be quick, simple, precise, and accurate and allowed for evaluating the compliance of cereals samples with legislated maximum residue levels of pesticides in the European Union. MDPI 2019-12-24 /pmc/articles/PMC7022397/ /pubmed/31878165 http://dx.doi.org/10.3390/foods9010018 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Melo, Maria Graça
Carqueijo, Ana
Freitas, Andreia
Barbosa, Jorge
Silva, Ana Sanches
Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.)
title Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.)
title_full Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.)
title_fullStr Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.)
title_full_unstemmed Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.)
title_short Modified QuEChERS Extraction and HPLC-MS/MS for Simultaneous Determination of 155 Pesticide Residues in Rice (Oryza sativa L.)
title_sort modified quechers extraction and hplc-ms/ms for simultaneous determination of 155 pesticide residues in rice (oryza sativa l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022397/
https://www.ncbi.nlm.nih.gov/pubmed/31878165
http://dx.doi.org/10.3390/foods9010018
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