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Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies

Four recombinant (r) allergens (rAmb a 8.0101, rArt v 4.0101, rBet v 2.0101, and rPhl p 12.0101) were successfully produced and used for sensitization studies. The allergens belong to the profilin family which is one of the most numerous allergen families. These four proteins represent allergens ori...

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Autores principales: Cudowska, Beata, Kapingidza, A. Brenda, Pawłowicz, Magdalena, Pampuch, Agnieszka, Hyduke, Noah, Pote, Swanandi, Schlachter, Caleb R., Lebensztejn, Dariusz M., Chruszcz, Maksymilian, Kowal, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024262/
https://www.ncbi.nlm.nih.gov/pubmed/31963206
http://dx.doi.org/10.3390/molecules25020369
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author Cudowska, Beata
Kapingidza, A. Brenda
Pawłowicz, Magdalena
Pampuch, Agnieszka
Hyduke, Noah
Pote, Swanandi
Schlachter, Caleb R.
Lebensztejn, Dariusz M.
Chruszcz, Maksymilian
Kowal, Krzysztof
author_facet Cudowska, Beata
Kapingidza, A. Brenda
Pawłowicz, Magdalena
Pampuch, Agnieszka
Hyduke, Noah
Pote, Swanandi
Schlachter, Caleb R.
Lebensztejn, Dariusz M.
Chruszcz, Maksymilian
Kowal, Krzysztof
author_sort Cudowska, Beata
collection PubMed
description Four recombinant (r) allergens (rAmb a 8.0101, rArt v 4.0101, rBet v 2.0101, and rPhl p 12.0101) were successfully produced and used for sensitization studies. The allergens belong to the profilin family which is one of the most numerous allergen families. These four proteins represent allergens originating from pollen of weeds (rAmb a 8.0101 and rArt v 4.0101), tree (rBet v 2.0101) and grass (rPhl p 12.0101). The recombinant allergens were characterized using various biochemical and biophysical methods and tested for their ability to bind patient-derived antibodies. One hundred patients aged 2 to 50 years sensitized to pollen and plant-derived food allergens (IgE > 0.35 kU/L) were included. Sensitization to individual allergen sources and components of birch and timothy pollens was evaluated using multiparameter immunoblots. The presence of IgE to pollen-derived recombinant profilins rAmb a 8.0101, rArt v 4.0101, rBet v 2.0101, and rPhl p 12.0101 in serum was evaluated using ELISA method. The presence of IgE against pollen profilins was detected in 20 out of 100 studied patients. High correlation was seen between IgE ELISA results with individual pollen profilins. In summary, it was shown that the recombinant versions of the four allergenic profilins can be used for sensitization studies and for component-resolved allergy diagnostics.
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spelling pubmed-70242622020-03-11 Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies Cudowska, Beata Kapingidza, A. Brenda Pawłowicz, Magdalena Pampuch, Agnieszka Hyduke, Noah Pote, Swanandi Schlachter, Caleb R. Lebensztejn, Dariusz M. Chruszcz, Maksymilian Kowal, Krzysztof Molecules Article Four recombinant (r) allergens (rAmb a 8.0101, rArt v 4.0101, rBet v 2.0101, and rPhl p 12.0101) were successfully produced and used for sensitization studies. The allergens belong to the profilin family which is one of the most numerous allergen families. These four proteins represent allergens originating from pollen of weeds (rAmb a 8.0101 and rArt v 4.0101), tree (rBet v 2.0101) and grass (rPhl p 12.0101). The recombinant allergens were characterized using various biochemical and biophysical methods and tested for their ability to bind patient-derived antibodies. One hundred patients aged 2 to 50 years sensitized to pollen and plant-derived food allergens (IgE > 0.35 kU/L) were included. Sensitization to individual allergen sources and components of birch and timothy pollens was evaluated using multiparameter immunoblots. The presence of IgE to pollen-derived recombinant profilins rAmb a 8.0101, rArt v 4.0101, rBet v 2.0101, and rPhl p 12.0101 in serum was evaluated using ELISA method. The presence of IgE against pollen profilins was detected in 20 out of 100 studied patients. High correlation was seen between IgE ELISA results with individual pollen profilins. In summary, it was shown that the recombinant versions of the four allergenic profilins can be used for sensitization studies and for component-resolved allergy diagnostics. MDPI 2020-01-16 /pmc/articles/PMC7024262/ /pubmed/31963206 http://dx.doi.org/10.3390/molecules25020369 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cudowska, Beata
Kapingidza, A. Brenda
Pawłowicz, Magdalena
Pampuch, Agnieszka
Hyduke, Noah
Pote, Swanandi
Schlachter, Caleb R.
Lebensztejn, Dariusz M.
Chruszcz, Maksymilian
Kowal, Krzysztof
Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies
title Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies
title_full Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies
title_fullStr Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies
title_full_unstemmed Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies
title_short Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies
title_sort production and use of recombinant profilins amb a 8, art v 4, bet v 2, and phl p 12 for allergenic sensitization studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024262/
https://www.ncbi.nlm.nih.gov/pubmed/31963206
http://dx.doi.org/10.3390/molecules25020369
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