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Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings
The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five Listeria monocytogenes isolates from a varie...
Главные авторы: | , , , , , , |
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Формат: | Online Статья Текст |
Язык: | English |
Опубликовано: |
MDPI
2020
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Предметы: | |
Online-ссылка: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024322/ https://www.ncbi.nlm.nih.gov/pubmed/31963433 http://dx.doi.org/10.3390/molecules25020383 |
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author | Cusimano, Maria Grazia Di Stefano, Vita La Giglia, Maria Di Marco Lo Presti, Vincenzo Schillaci, Domenico Pomilio, Francesco Vitale, Maria |
author_facet | Cusimano, Maria Grazia Di Stefano, Vita La Giglia, Maria Di Marco Lo Presti, Vincenzo Schillaci, Domenico Pomilio, Francesco Vitale, Maria |
author_sort | Cusimano, Maria Grazia |
collection | PubMed |
description | The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five Listeria monocytogenes isolates from a variety of foods and five Escherichia coli isolates from a farm. The E. coli isolates were positive for extended spectrum β-lactamase (ESBL) genes. All isolates and reference strains were susceptible to thymol at Minimum inhibitory concentration (MIC) values ranging from 250 to 800 μg/mL. An interesting activity of interference with biofilm formation of L. monocytogenes and E. coli was found for thymol at sub-MIC concentrations of 200, 100, 75, and 50 μg/mL. Anti-biofilm activity ranging from 59.71% to 66.90% against pre-formed 24-h-old L. monocytogenes biofilms at concentrations of 500 or 800 µg/mL, corresponding to 2× MIC, was determined against free-living forms of six isolates chosen as the best or moderate biofilm producers among the tested strains. The property of thymol to attack L. monocytogenes biofilm formation was also observed at a concentration of 100 µg/mL, corresponding to 1/4 MIC, by using a stainless-steel model to simulate the surfaces in food industries. This study gives information on the use of thymol in food processing setting. |
format | Online Article Text |
id | pubmed-7024322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70243222020-03-11 Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings Cusimano, Maria Grazia Di Stefano, Vita La Giglia, Maria Di Marco Lo Presti, Vincenzo Schillaci, Domenico Pomilio, Francesco Vitale, Maria Molecules Article The main objective of this study was to evaluate the efficacy of thymol in controlling environmental contamination in food processing facilities. The effect of thymol was tested as an agent to prevent planktonic and bacterial biofilm growth of twenty-five Listeria monocytogenes isolates from a variety of foods and five Escherichia coli isolates from a farm. The E. coli isolates were positive for extended spectrum β-lactamase (ESBL) genes. All isolates and reference strains were susceptible to thymol at Minimum inhibitory concentration (MIC) values ranging from 250 to 800 μg/mL. An interesting activity of interference with biofilm formation of L. monocytogenes and E. coli was found for thymol at sub-MIC concentrations of 200, 100, 75, and 50 μg/mL. Anti-biofilm activity ranging from 59.71% to 66.90% against pre-formed 24-h-old L. monocytogenes biofilms at concentrations of 500 or 800 µg/mL, corresponding to 2× MIC, was determined against free-living forms of six isolates chosen as the best or moderate biofilm producers among the tested strains. The property of thymol to attack L. monocytogenes biofilm formation was also observed at a concentration of 100 µg/mL, corresponding to 1/4 MIC, by using a stainless-steel model to simulate the surfaces in food industries. This study gives information on the use of thymol in food processing setting. MDPI 2020-01-17 /pmc/articles/PMC7024322/ /pubmed/31963433 http://dx.doi.org/10.3390/molecules25020383 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cusimano, Maria Grazia Di Stefano, Vita La Giglia, Maria Di Marco Lo Presti, Vincenzo Schillaci, Domenico Pomilio, Francesco Vitale, Maria Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings |
title | Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings |
title_full | Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings |
title_fullStr | Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings |
title_full_unstemmed | Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings |
title_short | Control of Growth and Persistence of Listeria monocytogenes and β-Lactam-Resistant Escherichia coli by Thymol in Food Processing Settings |
title_sort | control of growth and persistence of listeria monocytogenes and β-lactam-resistant escherichia coli by thymol in food processing settings |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024322/ https://www.ncbi.nlm.nih.gov/pubmed/31963433 http://dx.doi.org/10.3390/molecules25020383 |
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