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Single-molecule imaging analysis reveals the mechanism of a high-catalytic-activity mutant of chitinase A from Serratia marcescens

Chitin degradation is important for biomass conversion and has potential applications for agriculture, biotechnology, and the pharmaceutical industry. Chitinase A from the Gram-negative bacterium Serratia marcescens (SmChiA) is a processive enzyme that hydrolyzes crystalline chitin as it moves linea...

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Detalles Bibliográficos
Autores principales: Visootsat, Akasit, Nakamura, Akihiko, Vignon, Paul, Watanabe, Hiroki, Uchihashi, Takayuki, Iino, Ryota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029130/
https://www.ncbi.nlm.nih.gov/pubmed/31924658
http://dx.doi.org/10.1074/jbc.RA119.012078