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Efficient induction of haploid plants in wheat by editing of TaMTL using an optimized Agrobacterium-mediated CRISPR system

The use of CRISPR/LbCpf1 and CRISPR/xCas9 systems in wheat have not yet been reported. In this study, we compared the efficiencies of three CRISPR editing systems (SpCas9, LbCpf1, and xCas9), and three different promoters (OsU6a, TaU3, and TaU6) that drive single-guide (sg)RNA, which were introduced...

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Detalles Bibliográficos
Autores principales: Liu, Huiyun, Wang, Ke, Jia, Zimiao, Gong, Qiang, Lin, Zhishan, Du, Lipu, Pei, Xinwu, Ye, Xingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031065/
https://www.ncbi.nlm.nih.gov/pubmed/31760434
http://dx.doi.org/10.1093/jxb/erz529