Golden Gate assembly with a bi-directional promoter (GBid): A simple, scalable method for phage display Fab library creation

Fabs offer an attractive platform for monoclonal antibody discovery/engineering, but library construction can be cumbersome. We report a simple method – Golden Gate assembly with a bi-directional promoter (GBid) – for constructing phage display Fab libraries. In GBid, the constant domains of the Fab...

Descripción completa

Detalles Bibliográficos
Autores principales: Chockalingam, Karuppiah, Peng, Zeyu, Vuong, Christine N., Berghman, Luc R., Chen, Zhilei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031318/
https://www.ncbi.nlm.nih.gov/pubmed/32076016
http://dx.doi.org/10.1038/s41598-020-59745-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031318/
https://www.ncbi.nlm.nih.gov/pubmed/32076016
http://dx.doi.org/10.1038/s41598-020-59745-2

Ejemplares similares